Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation

Ernazarov Zafarjon Mamurovich; Pozilov Mamurjon Komiljonovich; Toshmatova Shoiraxon Ruziyevna; Zhurakulov Sherzod Niyatkobulovich

doi:doi:10.11648/j.ajbls.20221003.12

Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation

Ernazarov Zafarjon Mamurovich, Pozilov Mamurjon Komiljonovich, Toshmatova Shoiraxon Ruziyevna, Zhurakulov Sherzod Niyatkobulovich

Published in American Journal of Biomedical and Life Sciences (Volume 10, Issue 3)

Received: 9 April 2022 Accepted: 5 May 2022 Published: 24 May 2022

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Abstract

In current scientific article, rat liver mitochondria respond to the flavonoids dihydroquercetin (20–100 µM), 1-(2´-bromo-4´,5´-dimethoxyphenyl)-6,7-dimethoxy-1,2,3,4 for respiratory and processes of oxidative phosphorylation - tetrahydroisoquinolines F-18 (20-100 μM) and synthesized on the basis of followings 2-(3,4-dihydroxyphenyl)-6-[1-(2'-bromo-4',5'-dimethoxyphenyl)-6,7- The effect of dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl]methyl-3,5,7-trihydroxychroman-4-on the concentrations of the DHQ-11 conjugate (20-100 μM) was investigated in in vitro experiments. Experiments were conducted within 180-200 gram infertile white male rats. Mitochondrias from rat liver were isolated by the way of differential centrifugation. Concentrations of dihydroquercetin flavonoid 20, 60, 100 μM in FAD-dependent succinate oxidation in rat liver mitochondria were identified to be reliable respiratory control parameters and partially increased ADF/O value, the respiratory control coefficient as Chans demonstrated an increase in respiratory rate under the influence of the alkaloid F-18 isoquinoline 20, 60, 100 μM. Moreover, the ADP/O coefficients also boosted under the influence of the isoquinoline alkaloid F-18. It was observed that ADP/O ratio coefficients was expanded within the bonding by the effect of the DHQ-11 conjugate concentration. The concentration of 100 μM dihydroquercetin for FAD-dependent succinate oxidation in rat liver mitochondria surged Chance's respiratory rate by 15% compared to the control. Concentrations of isoquinoline alkaloid F-18 100 μM enlarged the respiratory rate by 13% compared to the control. It was defined that concentrations of 100 μM DHQ-11 conjugate rose the respiratory rate by 20%. The oxidation of FAD-dependent substrates in mitochondria was more actively effected by the DHQ-11 conjugate than dihydroquercetin and F-18 isoquinoline alkaloids.

Published in	American Journal of Biomedical and Life Sciences (Volume 10, Issue 3)
DOI	10.11648/j.ajbls.20221003.12
Page(s)	65-69
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Liver, Mitochondria, FAD, ROS, Succinate, Dihydroquercetin, F-18 Isoquinoline Alkaloid, DHQ-11 Conjugate

References

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[3]	Biasutto L, Sassi N, Mattarei A, Marotta E, Cattelan P, Toninello A, et al. Impact of mitochondriotropic quercetin derivatives on mitochondria. Biochim. Biophys. Acta 2010; 1797: 189–196. pmid: 19835835.
[4]	Bridges H. R., Jones A. Y., Pollak M. N., Hirst J. Effects of metformin and other biguanides on oxidative phosphorylation in mitochondria // Biochem. J. - 2014. - V. 462. - P. 475-487.
[5]	Cao, G.; Sophich, E.; Prior, R. L. (1997) Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radik Biol Med. 749–760.
[6]	Drahota Z., Palenickova E., Endlicher R. et al. Biguanides inhibit complex I, II and IV of rat liver mitochondria and modify their functional properties // Physiol. Res. – 2014. - V. 63 (1). - P. 1-11.
[7]	Dzhumaev I. Z., Usmanov P. B., Zhurakulov Sh. N., Vinogradova V. I. (2019) Description of the mechanism of action of alkaloids F-24 and H-14 on the Ca²⁺ transport systems of cardiomyocytes // Infection, Immunity and Pharmacology No. 3 P. 21-27Dzhumaev I., Usmanov P., Rustamov S., Zhurakulov S.(2020) Comparative inotropic effects of some isoquinoline alkaloids. Biomed Pharmacol J. 13 (1).
[8]	Hernandez E.-Aquino and P. Muriel, (2018) Benefits of Naringenin in Liver Disease: Molecular Mechanisms, World Journal of Gastroenterology, vol. 24, no. 16, p. 1679–1707.
[9]	Kichinska A., Yarmushkevich V. (2020) Flavonoids and mitochondria: activation of cytoprotective pathways? Molecules 3060 8 out of 22.
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[11]	Usmanov P., Dzhumaev I., Rustamov S., Zaripov A., Esimbetov A., Zhurakulov S., Vinogradova V. (2021) Combined inotropic and vasodilating effect of DHA-11, dihydroquercetin flavonoid conjugate with isoquinoline alkaloid 1-aryl-6, 7-dimethoxy-1,2,3,4-tetrahydroisoquinoline. Biomed Pharmacol J; Volume. 14 (2), p. 651-661.
[12]	Voyer P. D. The ATP synthase – a splendid molecular machine // Ann. Rev. Biochem, 1997; V. 66: P. 717-749.
[13]	Weidmann A. E. (2012) Dihydroquercetin: more than just an impurity? European Journal of Pharmacology. Vol. 684, no. 1-3, p. 19-26.
[14]	Zhurakulov Sh. N., Babkin V. A., Chernyak E. I., Morozov S. V., Grigorev I. A., Levkovich M. G. and Vinogradova V. I. (2015) Aminomethylation of 1-aryl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinolines by dihydroquercetin. Chemistry of natural compounds. T, 51, №1, 57-61.

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Ernazarov Zafarjon Mamurovich, Pozilov Mamurjon Komiljonovich, Toshmatova Shoiraxon Ruziyevna, Zhurakulov Sherzod Niyatkobulovich. (2022). Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation. American Journal of Biomedical and Life Sciences, 10(3), 65-69. https://doi.org/10.11648/j.ajbls.20221003.12

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Ernazarov Zafarjon Mamurovich; Pozilov Mamurjon Komiljonovich; Toshmatova Shoiraxon Ruziyevna; Zhurakulov Sherzod Niyatkobulovich. Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation. Am. J. Biomed. Life Sci. 2022, 10(3), 65-69. doi: 10.11648/j.ajbls.20221003.12

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Ernazarov Zafarjon Mamurovich, Pozilov Mamurjon Komiljonovich, Toshmatova Shoiraxon Ruziyevna, Zhurakulov Sherzod Niyatkobulovich. Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation. Am J Biomed Life Sci. 2022;10(3):65-69. doi: 10.11648/j.ajbls.20221003.12

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@article{10.11648/j.ajbls.20221003.12,
  author = {Ernazarov Zafarjon Mamurovich and Pozilov Mamurjon Komiljonovich and Toshmatova Shoiraxon Ruziyevna and Zhurakulov Sherzod Niyatkobulovich},
  title = {Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {10},
  number = {3},
  pages = {65-69},
  doi = {10.11648/j.ajbls.20221003.12},
  url = {https://doi.org/10.11648/j.ajbls.20221003.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20221003.12},
  abstract = {In current scientific article, rat liver mitochondria respond to the flavonoids dihydroquercetin (20–100 µM), 1-(2´-bromo-4´,5´-dimethoxyphenyl)-6,7-dimethoxy-1,2,3,4 for respiratory and processes of oxidative phosphorylation - tetrahydroisoquinolines F-18 (20-100 μM) and synthesized on the basis of followings 2-(3,4-dihydroxyphenyl)-6-[1-(2'-bromo-4',5'-dimethoxyphenyl)-6,7- The effect of dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl]methyl-3,5,7-trihydroxychroman-4-on the concentrations of the DHQ-11 conjugate (20-100 μM) was investigated in in vitro experiments. Experiments were conducted within 180-200 gram infertile white male rats. Mitochondrias from rat liver were isolated by the way of differential centrifugation. Concentrations of dihydroquercetin flavonoid 20, 60, 100 μM in FAD-dependent succinate oxidation in rat liver mitochondria were identified to be reliable respiratory control parameters and partially increased ADF/O value, the respiratory control coefficient as Chans demonstrated an increase in respiratory rate under the influence of the alkaloid F-18 isoquinoline 20, 60, 100 μM. Moreover, the ADP/O coefficients also boosted under the influence of the isoquinoline alkaloid F-18. It was observed that ADP/O ratio coefficients was expanded within the bonding by the effect of the DHQ-11 conjugate concentration. The concentration of 100 μM dihydroquercetin for FAD-dependent succinate oxidation in rat liver mitochondria surged Chance's respiratory rate by 15% compared to the control. Concentrations of isoquinoline alkaloid F-18 100 μM enlarged the respiratory rate by 13% compared to the control. It was defined that concentrations of 100 μM DHQ-11 conjugate rose the respiratory rate by 20%. The oxidation of FAD-dependent substrates in mitochondria was more actively effected by the DHQ-11 conjugate than dihydroquercetin and F-18 isoquinoline alkaloids.},
 year = {2022}
}

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TY  - JOUR
T1  - Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation
AU  - Ernazarov Zafarjon Mamurovich
AU  - Pozilov Mamurjon Komiljonovich
AU  - Toshmatova Shoiraxon Ruziyevna
AU  - Zhurakulov Sherzod Niyatkobulovich
Y1  - 2022/05/24
PY  - 2022
N1  - https://doi.org/10.11648/j.ajbls.20221003.12
DO  - 10.11648/j.ajbls.20221003.12
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 65
EP  - 69
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20221003.12
AB  - In current scientific article, rat liver mitochondria respond to the flavonoids dihydroquercetin (20–100 µM), 1-(2´-bromo-4´,5´-dimethoxyphenyl)-6,7-dimethoxy-1,2,3,4 for respiratory and processes of oxidative phosphorylation - tetrahydroisoquinolines F-18 (20-100 μM) and synthesized on the basis of followings 2-(3,4-dihydroxyphenyl)-6-[1-(2'-bromo-4',5'-dimethoxyphenyl)-6,7- The effect of dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl]methyl-3,5,7-trihydroxychroman-4-on the concentrations of the DHQ-11 conjugate (20-100 μM) was investigated in in vitro experiments. Experiments were conducted within 180-200 gram infertile white male rats. Mitochondrias from rat liver were isolated by the way of differential centrifugation. Concentrations of dihydroquercetin flavonoid 20, 60, 100 μM in FAD-dependent succinate oxidation in rat liver mitochondria were identified to be reliable respiratory control parameters and partially increased ADF/O value, the respiratory control coefficient as Chans demonstrated an increase in respiratory rate under the influence of the alkaloid F-18 isoquinoline 20, 60, 100 μM. Moreover, the ADP/O coefficients also boosted under the influence of the isoquinoline alkaloid F-18. It was observed that ADP/O ratio coefficients was expanded within the bonding by the effect of the DHQ-11 conjugate concentration. The concentration of 100 μM dihydroquercetin for FAD-dependent succinate oxidation in rat liver mitochondria surged Chance's respiratory rate by 15% compared to the control. Concentrations of isoquinoline alkaloid F-18 100 μM enlarged the respiratory rate by 13% compared to the control. It was defined that concentrations of 100 μM DHQ-11 conjugate rose the respiratory rate by 20%. The oxidation of FAD-dependent substrates in mitochondria was more actively effected by the DHQ-11 conjugate than dihydroquercetin and F-18 isoquinoline alkaloids.
VL  - 10
IS  - 3
ER  -

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Author Information

Ernazarov Zafarjon Mamurovich

Department of Biophysics, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
Pozilov Mamurjon Komiljonovich

Department of Biophysics, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
Toshmatova Shoiraxon Ruziyevna

Department of Biology, Faculty of Natural Sciences, Kokand State Pedagogical Institute Named After Muqimi, Kokand, Uzbekistan
Zhurakulov Sherzod Niyatkobulovich

Department of Biophysics, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan

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APA Style

Ernazarov Zafarjon Mamurovich, Pozilov Mamurjon Komiljonovich, Toshmatova Shoiraxon Ruziyevna, Zhurakulov Sherzod Niyatkobulovich. (2022). Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation. American Journal of Biomedical and Life Sciences, 10(3), 65-69. https://doi.org/10.11648/j.ajbls.20221003.12

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ACS Style

Ernazarov Zafarjon Mamurovich; Pozilov Mamurjon Komiljonovich; Toshmatova Shoiraxon Ruziyevna; Zhurakulov Sherzod Niyatkobulovich. Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation. Am. J. Biomed. Life Sci. 2022, 10(3), 65-69. doi: 10.11648/j.ajbls.20221003.12

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AMA Style

Ernazarov Zafarjon Mamurovich, Pozilov Mamurjon Komiljonovich, Toshmatova Shoiraxon Ruziyevna, Zhurakulov Sherzod Niyatkobulovich. Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation. Am J Biomed Life Sci. 2022;10(3):65-69. doi: 10.11648/j.ajbls.20221003.12

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@article{10.11648/j.ajbls.20221003.12,
  author = {Ernazarov Zafarjon Mamurovich and Pozilov Mamurjon Komiljonovich and Toshmatova Shoiraxon Ruziyevna and Zhurakulov Sherzod Niyatkobulovich},
  title = {Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {10},
  number = {3},
  pages = {65-69},
  doi = {10.11648/j.ajbls.20221003.12},
  url = {https://doi.org/10.11648/j.ajbls.20221003.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20221003.12},
  abstract = {In current scientific article, rat liver mitochondria respond to the flavonoids dihydroquercetin (20–100 µM), 1-(2´-bromo-4´,5´-dimethoxyphenyl)-6,7-dimethoxy-1,2,3,4 for respiratory and processes of oxidative phosphorylation - tetrahydroisoquinolines F-18 (20-100 μM) and synthesized on the basis of followings 2-(3,4-dihydroxyphenyl)-6-[1-(2'-bromo-4',5'-dimethoxyphenyl)-6,7- The effect of dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl]methyl-3,5,7-trihydroxychroman-4-on the concentrations of the DHQ-11 conjugate (20-100 μM) was investigated in in vitro experiments. Experiments were conducted within 180-200 gram infertile white male rats. Mitochondrias from rat liver were isolated by the way of differential centrifugation. Concentrations of dihydroquercetin flavonoid 20, 60, 100 μM in FAD-dependent succinate oxidation in rat liver mitochondria were identified to be reliable respiratory control parameters and partially increased ADF/O value, the respiratory control coefficient as Chans demonstrated an increase in respiratory rate under the influence of the alkaloid F-18 isoquinoline 20, 60, 100 μM. Moreover, the ADP/O coefficients also boosted under the influence of the isoquinoline alkaloid F-18. It was observed that ADP/O ratio coefficients was expanded within the bonding by the effect of the DHQ-11 conjugate concentration. The concentration of 100 μM dihydroquercetin for FAD-dependent succinate oxidation in rat liver mitochondria surged Chance's respiratory rate by 15% compared to the control. Concentrations of isoquinoline alkaloid F-18 100 μM enlarged the respiratory rate by 13% compared to the control. It was defined that concentrations of 100 μM DHQ-11 conjugate rose the respiratory rate by 20%. The oxidation of FAD-dependent substrates in mitochondria was more actively effected by the DHQ-11 conjugate than dihydroquercetin and F-18 isoquinoline alkaloids.},
 year = {2022}
}

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TY  - JOUR
T1  - Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation
AU  - Ernazarov Zafarjon Mamurovich
AU  - Pozilov Mamurjon Komiljonovich
AU  - Toshmatova Shoiraxon Ruziyevna
AU  - Zhurakulov Sherzod Niyatkobulovich
Y1  - 2022/05/24
PY  - 2022
N1  - https://doi.org/10.11648/j.ajbls.20221003.12
DO  - 10.11648/j.ajbls.20221003.12
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 65
EP  - 69
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20221003.12
AB  - In current scientific article, rat liver mitochondria respond to the flavonoids dihydroquercetin (20–100 µM), 1-(2´-bromo-4´,5´-dimethoxyphenyl)-6,7-dimethoxy-1,2,3,4 for respiratory and processes of oxidative phosphorylation - tetrahydroisoquinolines F-18 (20-100 μM) and synthesized on the basis of followings 2-(3,4-dihydroxyphenyl)-6-[1-(2'-bromo-4',5'-dimethoxyphenyl)-6,7- The effect of dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl]methyl-3,5,7-trihydroxychroman-4-on the concentrations of the DHQ-11 conjugate (20-100 μM) was investigated in in vitro experiments. Experiments were conducted within 180-200 gram infertile white male rats. Mitochondrias from rat liver were isolated by the way of differential centrifugation. Concentrations of dihydroquercetin flavonoid 20, 60, 100 μM in FAD-dependent succinate oxidation in rat liver mitochondria were identified to be reliable respiratory control parameters and partially increased ADF/O value, the respiratory control coefficient as Chans demonstrated an increase in respiratory rate under the influence of the alkaloid F-18 isoquinoline 20, 60, 100 μM. Moreover, the ADP/O coefficients also boosted under the influence of the isoquinoline alkaloid F-18. It was observed that ADP/O ratio coefficients was expanded within the bonding by the effect of the DHQ-11 conjugate concentration. The concentration of 100 μM dihydroquercetin for FAD-dependent succinate oxidation in rat liver mitochondria surged Chance's respiratory rate by 15% compared to the control. Concentrations of isoquinoline alkaloid F-18 100 μM enlarged the respiratory rate by 13% compared to the control. It was defined that concentrations of 100 μM DHQ-11 conjugate rose the respiratory rate by 20%. The oxidation of FAD-dependent substrates in mitochondria was more actively effected by the DHQ-11 conjugate than dihydroquercetin and F-18 isoquinoline alkaloids.
VL  - 10
IS  - 3
ER  -

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