DNA methylation is an epigenetic mechanism involved in the regulation of blood lipid levels which contribute to the cardiovascular risk in diabetic patients. Active agents that target atherogenic dyslipidemias epigenetically are therefore of paramount interest for prevention of cardiovascular complications in these patients. This study aims at evaluating the antiatherogenic effect of hydroethanolic extract of A. congolensis (HEEAC) on diabetic rats. Diabetes was induced by a single dose of 50 mg/kg b.w streptozotocin. Diabetic rats were then treated with 150 mg/kg b.w of HEEAC or with atorvastatin 10 mg/kg b.w (reference drug) for 28 days. At the end of the experimental period, rats were sacrificed, blood samples and liver tissues were collected and the plasma concentrations of triglycerides (TG), total cholesterol (TC), HDL-cholesterol and LDL-cholesterol were assessed. The atherogenic indices were also calculated and the liver and blood DNA extracted to determine DNA methylation. Comparing to untreated diabetic rats, the HEEAC treated group showed significant lower values of TG 245.98 ± 41.39 vs 57.88 ± 10.64 mg/dL (p < 0.05), TC 159.88 ± 17.56 vs. 87.77 ± 9.51 mg/dL (p < 0.05), LDL-C 94.51 ± 0.66 vs 48.71 ± 1.45 mg/dL (p < 0.05) and higher values of HDL-C 19.55 ± 1.6 vs 27.49 ± 1.45 (p < 0.05). Atherogenic indices were significantly lowered in HEEAC-group. The effects of HEEAC on lipid profile and atherogenic indices were significantly higher than atorvastatin. Also, the percentage of global hepatic DNA methylation (0.34±0.033 vs 0.63±0.023 %) was significantly increased in HEEAC-group compared to untreated diabetic group. DNA methylation profile in HEEAC-group correlated negatively and significantly (P < 0.01) with LDL-C and TC levels. HEEAC prevents atherogenic dyslipidemia in diabetic rats by targeting global DNA-methylation status in diabetic rats.
| Published in | American Journal of Biomedical and Life Sciences (Volume 11, Issue 3) |
| DOI | 10.11648/j.ajbls.20231103.14 |
| Page(s) | 53-59 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Diabetes, DNA Methylation, Liver, Lipid Profile, Atherogenic Risk, Autranella congolensis
| [1] | American Diabetes Association (2020). Classification and Diagnosis of Diabetes: Standards of Medical Care in Diabetes-2020. Diabetes Care, 43 Suppl 1: S14‑S31. https://doi.org/10.2337/dc20-S002 57 |
| [2] | Canto, ED., Ceriello, A., Ryde, L., Ferrini, M., Hansen, TB., Schnell, O., Standl, E., Beulens, WJ (2019). Diabetes as a cardiovascular risk factor: An overview of global trends of macro and micro vascular complications. Eur J Prev Cardiol., 26 (2S): 25–32. |
| [3] | Pfeiffer, L., Wahl, S., Pilling, LC., Reischl, E., Sandling, JK., Sonja, Kunze., et al. (2015). DNA Methylation of Lipid-Related Genes Affects Blood Lipid Levels. Circ Cardiovasc Genet., 8 (2): 334–342. https://doi:10.1161/CIRCGENETICS.114.000804 |
| [4] | Gouri, A., Bouchareb, M., Dekaken, A., Bentorki, AA., Chefrour, M., Guieu, R., Benharkat, S. (2015). Epigenetics and pathogenesis of type 2 diabetes. Batna J Med Sci., 2: x-x4. |
| [5] | Kirchner, H., Sinha, I., Gao, H., Ruby, M., Schönke, M., Lindvall, J., et al. (2016). Altered DNA methylation of glycolytic and lipogenic genes in liver from obese and type 2 diabetic patients. Mol Metab., 5: 171-183. |
| [6] | Mortusewicz, O., Schermelleh, L., Walter, J., Cardoso, MC., Leonhardt, H. (2005). Recruitment of DNA methyltransferase I to DNA repair sites. Proc Natl Acad Sci USA.; 102: 8905–8909. |
| [7] | Allis, CD., Jenuwein, T. (2016). The molecular hallmarks of epigenetic control. Nature Reviews. Genetics, 17 (8): 487‑500. https://doi.org/10.1038/nrg.2016.59 |
| [8] | Guay, C., Regazzi, R. (2013). Circulating microRNAs as novel biomarkers for diabetes mellitus. Nat Rev Endocrinol., 9 (9): 513‑521. |
| [9] | Shanak, S., Saad, B., Hilal, Z. (2019). Metabolic and Epigenetic Action Mechanisms of Antidiabetic Medicinal Plants. Evid-Based Complementary and Altern Med., 2019: 18. https://doi.org/10.1155/2019/3583067 |
| [10] | Khan, MI., Rath, S., Adhami, VM., Mukhtar, H. (2018). Targeting epigenome with dietary nutrients in cancer: current advances and future challenges. Pharmacol Res., 129: 375–387. |
| [11] | Hardman, WE. (2014). Diet components can suppress inflammation and reduce cancer risk. Nutr Res Pract., 8 (3): 233-240. |
| [12] | Takuissu, NGR., Ngondi, JL., Oben, JE. (2020). Antioxidant and Glucose Lowering Effects of Hydroethanolic Extract of Baillonella toxisperma Pulp. JFR. 9 (2): 20. https://doi.org/10.5539/jfr.v9n2p20 12 |
| [13] | Ngoumen, NDJ., Ngondi, JL., Oben, JE. (2020). Effect of Autranella congolensis on Lipid Profile of Rats’ Brain with Experimentally Induced Alzheimer’s disease. JFR., 9 (4): 60-70. https://doi.org/10.5539/jfr.v9n4p60 |
| [14] | Al-Shamaony, L., Al-Khazraji, SM., Twaij, HAA. (1994). Hypoglycaemic effect of Artemisia herba alba. II. Effect of a valuable extract on some blood parameters in diabetic animals. J Ethnopharmacol., 43 (3): 167-171. https://doi.org/10.1016/0378-8741(94)90038-8 |
| [15] | Coombs, NJ., Gough, AG., Primrose, JN. (1999). Optimization of DNA and RNA extraction from archival formalin-fixed tissue. Nucleic acids Res., 27 (16). |
| [16] | Friedewald, W., Levy, R., Friedrickson, D. (1972). Estimation of the concentration of low density lipoprotein cholesterol in plasma, without the use of the preparative ultracentrifuge. Clin Chem., 18 (6): 499-502. |
| [17] | Ikewuchi, J., Ikewuchi, C. (2009a). Alteration of plasma lipid profiles and atherogenic indices by Stachytarpheta jamaicensis L. (Vahl). Biochem., 21 (2): 71-77. |
| [18] | Ikewuchi, J., Ikewuchi, C. (2009b). Alteration of plasma lipid profile and atherogenic indices of cholesterol loaded rats by Tridax procumbens Linn: Implications for the management of obesity and cardiovascular diseases. Biochem., 21 (2): s95-99. |
| [19] | Ouchi, G., Komiya, I., Taira, S., Wakugami, T., Ohya, Y. (2022). Lipids Health Dis., 21:4 https://doi.org/10.1186/s12944-021-01612-8 |
| [20] | Reitman, Frankel EN. (1957). Lipid oxidation. Program of Lipid Research, 19: 1-22. |
| [21] | Yagi, K. (1976). Simple fluorometric assay for lipoperoxyde in blood plasma. Biochem. Med. 15: 212-216. |
| [22] | Sinha, K. (1972). Colorimetric assay of catalase. Anal. Biochem., 47: 389-394. |
| [23] | Misra, P., Fridovich, I. (1972). The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase. JB, 247: 3170-3175. |
| [24] | Wiklund, O., Håversen, L., Pettersson, C., Hultén, LM. (2007). How can we prevent cardiovascular disease in diabetes. J Intern Med., 262: 199-207. |
| [25] | Gadi, R., Samaha, FF. (2007). Dyslipidemia in type 2 diabetes mellitus. Curr Diab Rep., 7: 228-234. |
| [26] | Fonkoua, M., Tazon, WA., Ntentié, FR., Azantsa, B., Takuissu, GR., Ngondi, JL., Oben, JE. (2022). Aqueous Extract of Alstonia boonei Bark Reduces Chronic Hyperglycemia and Prevents its Complications through Increase of Hepatic Global Dna Methylation in Diabetic Wistar Rats. EJMP., 32 (12): 1-15. https://doi.org/10.1186/s12944-021-01612-8 |
| [27] | Anderson, OS., Sant, KE., Dolinoy, DC. (2012). Nutrition and epigenetics: An interplay of dietary methyl donors, one-carbon metabolism and DNA methylation. J Nutr Biochem., 23 (8): 853‑859. https://doi.org/10.1016/j.jnutbio.2012.03.003 |
| [28] | Zhang, JS., Lei, JP., Wei, GQ., Chen H, Ma CY, Jiang, HZ. (2016). Natural fatty acid synthase inhibitors as potent therapeutic agents for cancers: A review. Pharm Biol., 54 (9): 1919‑1925. https://doi.org/10.3109/13880209.2015.1113995 |
| [29] | Donohoe, DR., Bultman, SJ. (2012). Metaboloepigenetics: interrelationships between energy metabolism and epigenetic control of gene expression. J Cell Physiol. 2012; 227: 3169–3177. |
| [30] | Pop, S., Enciu, AM., Tarcomnicu I, Gille E, Tanase C. (2019). Phytochemicals in cancer prevention: modulating epigenetic alterations of DNA methylation. Phytochem Rev., 18: 1005–1024. https://doi.org/10.1007/s11101-019-09627-x (0123456789 |
| [31] | American Diabetes Association (2017). Standards of Medical Care in Diabetes 2017. Diabetes Care.0 (1): 1-80, 28. |
| [32] | Farias-Pereira, R., Park, CS., Park, Y. (2019). Mechanisms of action of coffee bioactive components on lipid metabolism. Food Sci Biotechnol., 28 (5): 1287‑1296. https://doi.org/10.1007/s10068-019-00662-0 |
APA Style
Maboune, N., Fonkoua, M., Goda, D., Ngoumen, D. J. N., Nanhah, J. K., et al. (2023). Autranella congolensis Extract Prevents Atherogenic Dyslipidemia in Diabetic Rats via Modulation of Global Hepatic DNA Methylation. American Journal of Biomedical and Life Sciences, 11(3), 53-59. https://doi.org/10.11648/j.ajbls.20231103.14
ACS Style
Maboune, N.; Fonkoua, M.; Goda, D.; Ngoumen, D. J. N.; Nanhah, J. K., et al. Autranella congolensis Extract Prevents Atherogenic Dyslipidemia in Diabetic Rats via Modulation of Global Hepatic DNA Methylation. Am. J. Biomed. Life Sci. 2023, 11(3), 53-59. doi: 10.11648/j.ajbls.20231103.14
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author = {Nafissatou Maboune and Martin Fonkoua and David Goda and Dany Joël Ngassa Ngoumen and Jules Kamga Nanhah and Bruno Dupon Ambamba Akamba and Jean Paul Chedjou and Maxwell Wandji Nguedjo and Fils Armand Ella and Javeres Leonel Ntepe Mbah and Guy Roussel Takuissu Nguemto and Judith Laure Ngondi},
title = {Autranella congolensis Extract Prevents Atherogenic Dyslipidemia in Diabetic Rats via Modulation of Global Hepatic DNA Methylation},
journal = {American Journal of Biomedical and Life Sciences},
volume = {11},
number = {3},
pages = {53-59},
doi = {10.11648/j.ajbls.20231103.14},
url = {https://doi.org/10.11648/j.ajbls.20231103.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20231103.14},
abstract = {DNA methylation is an epigenetic mechanism involved in the regulation of blood lipid levels which contribute to the cardiovascular risk in diabetic patients. Active agents that target atherogenic dyslipidemias epigenetically are therefore of paramount interest for prevention of cardiovascular complications in these patients. This study aims at evaluating the antiatherogenic effect of hydroethanolic extract of A. congolensis (HEEAC) on diabetic rats. Diabetes was induced by a single dose of 50 mg/kg b.w streptozotocin. Diabetic rats were then treated with 150 mg/kg b.w of HEEAC or with atorvastatin 10 mg/kg b.w (reference drug) for 28 days. At the end of the experimental period, rats were sacrificed, blood samples and liver tissues were collected and the plasma concentrations of triglycerides (TG), total cholesterol (TC), HDL-cholesterol and LDL-cholesterol were assessed. The atherogenic indices were also calculated and the liver and blood DNA extracted to determine DNA methylation. Comparing to untreated diabetic rats, the HEEAC treated group showed significant lower values of TG 245.98 ± 41.39 vs 57.88 ± 10.64 mg/dL (p < 0.05), TC 159.88 ± 17.56 vs. 87.77 ± 9.51 mg/dL (p < 0.05), LDL-C 94.51 ± 0.66 vs 48.71 ± 1.45 mg/dL (p < 0.05) and higher values of HDL-C 19.55 ± 1.6 vs 27.49 ± 1.45 (p < 0.05). Atherogenic indices were significantly lowered in HEEAC-group. The effects of HEEAC on lipid profile and atherogenic indices were significantly higher than atorvastatin. Also, the percentage of global hepatic DNA methylation (0.34±0.033 vs 0.63±0.023 %) was significantly increased in HEEAC-group compared to untreated diabetic group. DNA methylation profile in HEEAC-group correlated negatively and significantly (P < 0.01) with LDL-C and TC levels. HEEAC prevents atherogenic dyslipidemia in diabetic rats by targeting global DNA-methylation status in diabetic rats.},
year = {2023}
}
TY - JOUR T1 - Autranella congolensis Extract Prevents Atherogenic Dyslipidemia in Diabetic Rats via Modulation of Global Hepatic DNA Methylation AU - Nafissatou Maboune AU - Martin Fonkoua AU - David Goda AU - Dany Joël Ngassa Ngoumen AU - Jules Kamga Nanhah AU - Bruno Dupon Ambamba Akamba AU - Jean Paul Chedjou AU - Maxwell Wandji Nguedjo AU - Fils Armand Ella AU - Javeres Leonel Ntepe Mbah AU - Guy Roussel Takuissu Nguemto AU - Judith Laure Ngondi Y1 - 2023/06/27 PY - 2023 N1 - https://doi.org/10.11648/j.ajbls.20231103.14 DO - 10.11648/j.ajbls.20231103.14 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 53 EP - 59 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20231103.14 AB - DNA methylation is an epigenetic mechanism involved in the regulation of blood lipid levels which contribute to the cardiovascular risk in diabetic patients. Active agents that target atherogenic dyslipidemias epigenetically are therefore of paramount interest for prevention of cardiovascular complications in these patients. This study aims at evaluating the antiatherogenic effect of hydroethanolic extract of A. congolensis (HEEAC) on diabetic rats. Diabetes was induced by a single dose of 50 mg/kg b.w streptozotocin. Diabetic rats were then treated with 150 mg/kg b.w of HEEAC or with atorvastatin 10 mg/kg b.w (reference drug) for 28 days. At the end of the experimental period, rats were sacrificed, blood samples and liver tissues were collected and the plasma concentrations of triglycerides (TG), total cholesterol (TC), HDL-cholesterol and LDL-cholesterol were assessed. The atherogenic indices were also calculated and the liver and blood DNA extracted to determine DNA methylation. Comparing to untreated diabetic rats, the HEEAC treated group showed significant lower values of TG 245.98 ± 41.39 vs 57.88 ± 10.64 mg/dL (p < 0.05), TC 159.88 ± 17.56 vs. 87.77 ± 9.51 mg/dL (p < 0.05), LDL-C 94.51 ± 0.66 vs 48.71 ± 1.45 mg/dL (p < 0.05) and higher values of HDL-C 19.55 ± 1.6 vs 27.49 ± 1.45 (p < 0.05). Atherogenic indices were significantly lowered in HEEAC-group. The effects of HEEAC on lipid profile and atherogenic indices were significantly higher than atorvastatin. Also, the percentage of global hepatic DNA methylation (0.34±0.033 vs 0.63±0.023 %) was significantly increased in HEEAC-group compared to untreated diabetic group. DNA methylation profile in HEEAC-group correlated negatively and significantly (P < 0.01) with LDL-C and TC levels. HEEAC prevents atherogenic dyslipidemia in diabetic rats by targeting global DNA-methylation status in diabetic rats. VL - 11 IS - 3 ER -
Department of Biochemistry, Faculty of Science, University of Yaounde 1, Yaounde, Cameroon
